Microplate Alamar Blue assay for detecting anti-tubercular action of Albizia amara leaves

Albizia amara (Roxb.) B. Boivin has been used historically for the treatment of septicaemia, malignancy, delirium, and convulsions. A decoction made from bark is to treat rheumatism, hemorrhage, some bleeding disorders during pregnancy, as well stomach pain nasal issues. active ingredients like flavonoids, alkaloids, steroids, phenolic compounds, tannins, according early phytochemical screening. Flavonoids compounds have shown play an important role in anti-tubercular activity previous research. This led us investigate whether or not this herb possessed properties. Mycobacterium tuberculosis was found be sensitive concentrations Alamar Blue dye low 25 μg/ml when activities petroleum ether, methanol, ethanolic extract were tested. The fractions comparable that streptomycin, pyrazinamide, ciprofloxacin. HPTLC flavonoids substances are present, which may explain can seen. Taking research on leaves one step further could lead development a natural medicine fights TB safe works well.